Journal: Oxidative Medicine and Cellular Longevity

Article Title: The Long Noncoding RNA Hotair Regulates Oxidative Stress and Cardiac Myocyte Apoptosis during Ischemia-Reperfusion Injury

doi: 10.1155/2020/1645249

Figure Lengend Snippet: Hotair expression is increased by BRD4 in response to I/R stimulation. (a) Hotair expression within the reperfused myocardium in the indicated time points ( n = 8). (b) Western blot images and the quantitative data of BRD4 in hearts subjected to ischemia for 30 minutes and reperfusion for additional 24 hours (I/R) ( n = 6). (c) Hotair expression in murine hearts after I/R injury with or without JQ1 treatment ( n = 8). (d) Hotair expression in H9c2 cells subjected to hypoxia for 2 hours and reoxygenation for the indicated time points ( n = 8). (e) Western blot images and the quantitative data of BRD4 in H9c2 cells subjected to hypoxia for 2 hours and reoxygenation for additional 24 hours (H/R) ( n = 6). (f) The efficiency of small interfering RNA against BRD4 (si Brd4 ) detected by PCR data ( n = 8). (g) Hotair expression in H9c2 cells after H/R stimulation with or without si Brd4 ( n = 8). Data are presented as mean ± standard deviation (SD). ∗ P < 0.05 versus the matched group.

Article Snippet: To notify the role of BRD4 in vitro, H9c2 cells were incubated with small interfering RNA against BRD4 (si Brd4 , 50 nM; #RSS338226, Thermo Fisher Scientific) using Lipofectamine RNAiMAX (Invitrogen) for 24 hours before H/R stimulation [ ].

Techniques: Expressing, Western Blot, Small Interfering RNA, Standard Deviation

Journal: Cancer Cell International

Article Title: Caveolin-2 is regulated by BRD4 and contributes to cell growth in pancreatic cancer

doi: 10.1186/s12935-020-1135-0

Figure Lengend Snippet: BRD4 is overexpressed in PC. a Compared to adjacent normal tissues, higher BRD4 mRNA expression in ten fresh samples of PC. The GAPDH expression was detected as the endogenous control. b Representative Immunohistochemical images of BRD4 expression, which was primarily localized in the cell nucleus. Higher BRD4 protein expression was found in PC tissues. c 5 of 7 PC cells lines, including AsPC-1, MIAPaCa-2, PANC-1, SW1990 and CFPAC-1 had higher BRD4 mRNA than levels observed in normal pancreatic ductal epithelial cells HPDE. The GAPDH expression was detected as the endogenous control for mRNA expression. d Higher BRD4 and CAV-2 protein expression in PC cell lines. BRD4 was positive correlated with CAV-2 expression. The asterisk indicates that p is less than 0.05

Article Snippet: BRD4 nc/siRNA/control/overexpression, as well as CAV-2 nc/siRNA/control/overexpression were all designed and purchased from GeneChem (Shanghai, China).

Techniques: Expressing, Control, Immunohistochemical staining

Journal: Cancer Cell International

Article Title: Caveolin-2 is regulated by BRD4 and contributes to cell growth in pancreatic cancer

doi: 10.1186/s12935-020-1135-0

Figure Lengend Snippet: BRD4 promotes PC cell proliferation, migration and invasion in vitro. a Western blot was used to confirm the efficacy of BRD4 knockdown in CFPAC-1 and PANC-1 cell lines. The transfection efficiency was determined at 24 h after transfection. The results showed that G1 cell cycle CDK4 and CDK6 were decreased, while p21 was increased in response to BRD4 knockdown. b Sulforhodamine B assays showed that both BRD4-si1 and BRD4-si2 group significantly suppressed cell proliferation compared to BRD4-nc group. c Plate clone formations showed that, in comparisons with BRD4-nc group, both BRD4-si1 and BRD4-si2 group significantly suppressed clone formations. d Migration assay showed that both BRD4-si1 and BRD4-si2 group significantly suppressed cell migration and invasion compared to BRD4-nc group. e Western blot was used to confirm the efficacy of BRD4 overexpression in su.86.86. f Sulforhodamine B assays showed that BRD4-over group significantly promoted cell proliferation compared to BRD4-ctrl group. g Migration assay showed that BRD4-over group significantly increased cell migration and invasion compared to BRD4-ctrl group. The asterisk indicates that p is less than 0.05

Article Snippet: BRD4 nc/siRNA/control/overexpression, as well as CAV-2 nc/siRNA/control/overexpression were all designed and purchased from GeneChem (Shanghai, China).

Techniques: Migration, In Vitro, Western Blot, Knockdown, Transfection, Over Expression

Journal: Cancer Cell International

Article Title: Caveolin-2 is regulated by BRD4 and contributes to cell growth in pancreatic cancer

doi: 10.1186/s12935-020-1135-0

Figure Lengend Snippet: BRD4 increases PC cell growth in vivo. a – c Subcutaneous tumor transplantation test showed that, compared with PANC-1/BRD4-nc, PANC-1/BRD4-sh1 and PANC-1/BRD4-sh2 groups had slower growth rate ( a ) and tumor weight ( b , c ). d Representative immunohistochemical images for BRD4, ki67 and cleaved Caspase3 expression. In comparisons with PANC-1/BRD4-nc, both PANC-1/BRD4-sh1 and PANC-1/BRD4-sh2 groups had lower BRD4 and ki67 expression. However, cleaved Caspase 3 was negatively expressed in all groups. Scale bar, 50 μm. The asterisk indicates that p is less than 0.05

Article Snippet: BRD4 nc/siRNA/control/overexpression, as well as CAV-2 nc/siRNA/control/overexpression were all designed and purchased from GeneChem (Shanghai, China).

Techniques: In Vivo, Transplantation Assay, Immunohistochemical staining, Expressing

Journal: Cancer Cell International

Article Title: Caveolin-2 is regulated by BRD4 and contributes to cell growth in pancreatic cancer

doi: 10.1186/s12935-020-1135-0

Figure Lengend Snippet: CAV-2 is a downstream target of BRD4. a RNA sequence was employed to identify the downstream target of BRD4. The results showed that there were 27 differentially expressed gene, 12 upregulations and 15 downregulation. Among them, the downregulation of CAV-2 was the second most. b , c The CAV-2 mRNA ( b ) and protein ( c ) level in both BRD4-si1 and BRD4-si2 group was down-regulated. d , e Compared to BRD4-ctrl group, the CAV-2 mRNA ( d ) and protein ( e ) in BRD4-over group was up-regulated. f Sulforhodamine B assays showed that both CAV-2-si1 and CAV-2-si2 group significantly suppressed cell proliferation compared to CAV-2-nc group. g Sulforhodamine B assays showed that CAV-2-over group significantly promoted cell proliferation compared to BRD4-ctrl group. h Migration assay showed that both CAV-2-si1 and CAV-2-si2 group did not significantly suppressed cell migration and invasion compared to CAV-2-nc group. i Migration assay showed that BRD4-over group did not significantly increased cell migration and invasion compared to BRD4-ctrl group. The asterisk indicates that p is less than 0.05

Article Snippet: BRD4 nc/siRNA/control/overexpression, as well as CAV-2 nc/siRNA/control/overexpression were all designed and purchased from GeneChem (Shanghai, China).

Techniques: Sequencing, Migration

Journal: Cancer Cell International

Article Title: Caveolin-2 is regulated by BRD4 and contributes to cell growth in pancreatic cancer

doi: 10.1186/s12935-020-1135-0

Figure Lengend Snippet: CAV-2 overexpression partially restores BRD4-siRNA mediated cell proliferation. a Western blot was employed to detect BRD4, CAV-2, PCNA, C-myc, CDK2, and pRb expression when cotransfected CFPAC-1 and PANC-1 cells with BRD4 nc/siRNA and CAV-2 ctrl/over plasmid. BRD4 decreased PCNA, C-myc, CDK2, and pRb expression. CAV-2 overexpression can partially reverse the protein expression of BRD4 suppression. b , c. Sulforhodamine B assays ( b ) and plate clone formations ( c ) showed that CAV-2 overexpression partially reversed decreased cell proliferation of BRD4 knockdown. d Cell cycle experiments showed that suppression of BRD4 induced G1 phase arrest. CAV-2 overexpression can partially reverse the effects of BRD4 knockdown. The asterisk indicates that p is less than 0.05

Article Snippet: BRD4 nc/siRNA/control/overexpression, as well as CAV-2 nc/siRNA/control/overexpression were all designed and purchased from GeneChem (Shanghai, China).

Techniques: Over Expression, Western Blot, Expressing, Plasmid Preparation, Knockdown

Journal: Cancer Cell International

Article Title: Caveolin-2 is regulated by BRD4 and contributes to cell growth in pancreatic cancer

doi: 10.1186/s12935-020-1135-0

Figure Lengend Snippet: BRD4 regulates CAV-2 transcriptional activity. a ChIP results showed that BRD4 can directly bind to the promoter region (0 ~ − 500 bp, − 500 ~ − 1000 bp and − 1000 ~ − 1500 bp) of CAV-2. b Compared to BRD4-nc group, BRD4-si1 exhibited a reduced BRD4 recruitment to CAV-2 promoter. c Luciferase reporter experiment suggested that BRD4 had transcriptional activity at the − 1500 ~ 0 bp promoter region of CAV-2. After interfering with BRD4 expression, the transcriptional activation of CAV-2 could be inhibited. The asterisk indicates that p is less than 0.05

Article Snippet: BRD4 nc/siRNA/control/overexpression, as well as CAV-2 nc/siRNA/control/overexpression were all designed and purchased from GeneChem (Shanghai, China).

Techniques: Activity Assay, Luciferase, Expressing, Activation Assay

Journal: Cancer Cell International

Article Title: Caveolin-2 is regulated by BRD4 and contributes to cell growth in pancreatic cancer

doi: 10.1186/s12935-020-1135-0

Figure Lengend Snippet: Correlation between the clinicopathological characteristics and BRD4 and CAV-2 expression in PC

Article Snippet: BRD4 nc/siRNA/control/overexpression, as well as CAV-2 nc/siRNA/control/overexpression were all designed and purchased from GeneChem (Shanghai, China).

Techniques: Expressing

Journal: Cancer Cell International

Article Title: Caveolin-2 is regulated by BRD4 and contributes to cell growth in pancreatic cancer

doi: 10.1186/s12935-020-1135-0

Figure Lengend Snippet: Association between BRD4 and CAV-2 expression and prognosis in PC patients. a The log-rank test showed that patients with PC tumors expressing high BRD4 levels had significantly lower OS than those expressing low BRD4. b The patients expressing high CAV-2 levels had significantly lower OS than those expressing low CAV-2. c The log-rank test showed that BRD4 (high)/CAV-2 (high) correlated with shorter OS of PC patients compare to that of other groups, including BRD4 (low)/CAV-2 (low), BRD4 (low)/CAV-2 (high) and BRD4 (high)/CAV-2 (low). d Serial histological sections suggested a positive correlation between BRD4 and CAV-2 expression

Article Snippet: BRD4 nc/siRNA/control/overexpression, as well as CAV-2 nc/siRNA/control/overexpression were all designed and purchased from GeneChem (Shanghai, China).

Techniques: Expressing

Journal: Cancer Cell International

Article Title: Caveolin-2 is regulated by BRD4 and contributes to cell growth in pancreatic cancer

doi: 10.1186/s12935-020-1135-0

Figure Lengend Snippet: Summary of univariate and multivariate Cox regression analysis of overall survival

Article Snippet: BRD4 nc/siRNA/control/overexpression, as well as CAV-2 nc/siRNA/control/overexpression were all designed and purchased from GeneChem (Shanghai, China).

Techniques:

Journal: Cancer Cell International

Article Title: Caveolin-2 is regulated by BRD4 and contributes to cell growth in pancreatic cancer

doi: 10.1186/s12935-020-1135-0

Figure Lengend Snippet: Correlation between BRD4 and CAV-2 expression in PC

Article Snippet: BRD4 nc/siRNA/control/overexpression, as well as CAV-2 nc/siRNA/control/overexpression were all designed and purchased from GeneChem (Shanghai, China).

Techniques: Expressing

Journal: Cancer Cell International

Article Title: Caveolin-2 is regulated by BRD4 and contributes to cell growth in pancreatic cancer

doi: 10.1186/s12935-020-1135-0

Figure Lengend Snippet: Comparisons with BRD4 and CAV-2 expression between PC and paired adjacent normal tissues

Article Snippet: BRD4 nc/siRNA/control/overexpression, as well as CAV-2 nc/siRNA/control/overexpression were all designed and purchased from GeneChem (Shanghai, China).

Techniques: Expressing